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Reference type: Journal
Authors: Sun BH, Ni XJ, Cao YH, Cao GQ
Article Title: Electrochemical sensor based on magnetic molecularly imprinted nanoparticles modified magnetic electrode for determination of Hb.
Publication date: 2017
Journal: Biosensors and Bioelectronics
Volume: 91
Page numbers: 354-358.
DOI: 10.1016/j.bios.2016.12.056
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566316313070

Abstract: A fast and selective electrochemical sensor for determination of hemoglobin (Hb) was developed based on magnetic molecularly imprinted nanoparticles modified on the magnetic glassy carbon electrode. The nanoparticles Fe3O4@SiO2 with a magnetic core and a molecularly imprinted shell had regular structures and good monodispersity. Hb could be determined directly by electrochemical oxidization with the modified electrode. A magnetic field increased electrochemical response to Hb by two times. Imprinting Hb on the surface of Fe3O4@SiO2 shortened the response time within 7 min. Under optimum conditions, the imprinting factor toward the non-imprinted sensor was 2.8, and the separation factor of Hb to horseradish peroxidase was 2.6. The oxidation peak current had a linear relationship with Hb concentration ranged from 0.005 mg/ml to 0.1 mg/ml with a detection limit (S/N =3) of 0.0010 mg/ml. The sensors were successfully applied to analysis of Hb in whole blood samples with recoveries between 95.7% and 105%
Template and target information: protein, hemoglobin, Hb
Author keywords: Magnetic molecularly imprinted nanoparticles, Hb, Magnetic electrode, Fe3O4@SiO2


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