MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Khan MAR, Cardoso ARA, Sales MGF, Merino S, Tomás JM, Rius FX, Riu J
Article Title: Artificial receptors for the electrochemical detection of bacterial flagellar filaments from Proteus mirabilis.
Publication date: 2017
Journal: Sensors and Actuators B: Chemical
Volume: 244
Page numbers: 732-741.
DOI: 10.1016/j.snb.2017.01.018
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400517300187

Abstract: In this paper for the first time we successfully detect bacterial flagellar filaments from Proteus mirabilis using molecularly imprinted artificial receptors. These receptors acted as a sensing layer of the biosensors, assembled by imprinting flagellar proteins onto a polymeric backbone of electropolymerized phenol. In short, flagellar filaments were absorbed onto a carbon support, phenol was electropolymerized around it through the carbon conductive matrix to create the protein molecular molds, and finally the flagellar proteins were removed by enzymatic and electrochemical action. Each removed flagellar protein gave rise to an imprinted site with eventual rebinding ability. Electrical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed to measure the interaction of flagellar filaments with the sensing layer assembled on commercial screen-printed electrodes, providing low detection limits, high precision and selectivity toward the targeted protein. The detection limit was 0.7 ng/mL by EIS and 0.9 ng/mL by SWV. The artificial receptors were further assembled on home-made paper-printed electrodes, with the three-electrode system printed on a paper substrate, offering the possibility of detecting flagellar filaments at as low as 0.6 ng/mL with a disposable and cost-effective portable device. To the best of our knowledge this is the first sensing device where molecularly imprinted artificial receptors are tailored on home-made electrode based on paper substrates with three electrodes assembled together, which is a suitable approach for the fabrication of easy and cost-effective tailored electrodes
Template and target information: bacterial flagellar filaments, Proteus mirabilis, protein
Author keywords: Molecularly imprinted polymers, artificial receptors, Flagellar filaments, Proteus mirabilis, Screen-printed electrodes, Paper-printed electrodes, electropolymerization, Disposable device


  Eat, sleep, imprint, repeat shirt  SMI logo mug  Man of proper-tea mug in green

Molecules Special Issue call      Appeal for information






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon