MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Wang LJ, Cao XM, Sun XL, Wang ST, Liu J, Hao ZH, Qu BH, Zhao SJ
Article Title: Rapid determination of 15 β2-agonists residues in swine urine by ultra performance liquid chromatography-tandem mass spectrometry coupled with molecularly imprinted solid phase extraction.
Publication date: 2016
Journal: Chinese Journal of Chromatography
Volume: 34
Issue: (3)
Page numbers: 240-248.
DOI: 10.3724/SP.J.1123.2015.11003
Alternative URL: http://www.chrom-china.com/EN/abstract/abstract14046.shtml

Abstract: An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method coupled with molecularly imprinted solid phase extraction (MISPE) for rapid determination of 15 β2-agonists residues in swine urine has been established. In order to obtain the optimized purification conditions, different pH values of loading, washing and elution solvents for the MISPE procedure were optimized. Finally, the swine urine samples (pH 7.0) were performed directly by MISPE column, then the column was washed with water, acetonitrile and 0.5%(v/v) acetic acid in acetonitrile, and eluted with 10%(v/v) acetic acid in methanol. Meanwhile, the parameters of UPLC-MS/MS have been optimized.The UPLC separation was performed on a BEH C18 column, and eluted with the mobile phase consisting of 0.1%(v/v) formic acid aqueous solution and acetonitrile with linear gradient program. The analysis was in ESI positive ion scan mode and multiple reaction monitoring (MRM) mode. Under the optimum conditions, the blank swine urine was spiked with different concentrations analytes and taken for analysis to evaluate the established method. To highlight the advantage of MISPE, specificity adsorption for the 15 β2-agonists in swine urine was investigate. The linear ranges were 0.1-20 μg/L for the 15 β2-agonists ( r 2 0.992). The limits of detection (LODs) and the limits of quantification (LOQs) were 0.03 and 0.1 μg/L, respectively. At the spiked levels of 0.1, 0.5 and 2.5 μg/L in blank samples, the mean recoveries were 65.6%-115.0% with the relative standard deviations (RSDs, n=6) of 0.57%-16.1%. Overall, the proposed method is simple, quick, reliable,sensitive, and can be applied in large scale supervision of illegal usage of the 15 β2-agonists.
Template and target information: β2-agonists
Author keywords: ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS, MS), Molecularly imprinted solid phase extraction (MISPE), β2-Agonists, Swine urine


  Eat, sleep, imprint, repeat shirt  SMI logo mug  Man of proper-tea mug

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner