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Reference type: Journal
Authors: Zhao T, Wang JP, He JL, Deng QL, Wang S
Article Title: One-step post-imprint modification achieve dual-function of glycoprotein fluorescent sensor by "Click Chemistry".
Publication date: 2017
Journal: Biosensors and Bioelectronics
Volume: 91
Page numbers: 756-761.
DOI: 10.1016/j.bios.2017.01.046
Alternative URL: https://www.researchgate.net/publication/312603965_One-step_post-imprint_modification_achieve_dual-function_of_glycoprotein_fluorescent_sensor_by_Click_Chemistry

Abstract: A novel molecular imprinting fluorescent sensor synthesized avoiding complicated design and synthesis of a fluorescent functional monomer, and without the extra cost of additional fluorescence materials (quantum dots, carbon dots and fluorescein), only by a click reaction making the common protein imprinting polymers upgraded to a fluorescent sensing material which can be applied to the fast detection of glycoproteins. In this study, a common protein imprinted polymer (protein-IP) which containing a disulfide bond in the imprinted cavities was first synthesized, then the disulfide linkage was cleaved by reduction, the exposed thiol groups were used for the click reaction to introduce 4-vinyl phenylboronic acid to improve the imprinted recognition for glycoproteins and convert recognition behavior into fluorescent signal simultaneously in one-step site-specific post-imprinting modifications (PIMs). Good linear relationship was obtained in the range of 0.1-10 mg mL-1 by fluorescence assay, comparison of the adsorption capacity of the MIPs before and after modification proved that the method has no negative impact on the imprinting effect and exhibit good imprinting effect for glycoproteins of different molecular size and weight
Template and target information: protein, glycoprotein, horseradish peroxidase, HRP, ovalbumin, OVA, bovine serum albumin, BSA
Author keywords: Post-imprinting modifications, click chemistry, molecularly imprinted polymer, glycoprotein


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