Abstract: A new analytical method was developed for the simultaneous determination of 20 triazine and sulfonylurea herbicides residues in foods of plant origin by composite molecular imprinted polymer solid phase extraction (CMISPE) with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS /MS). The samples were extracted with acetonitrile and then cleaned with liquid-liquid extraction and composite molecular imprinting solid phase extraction. The target compounds were separated with Agilent ZORBAX RX-C8column (150 x 2.1 mm, 5μm). The mobile phase A (acetonitrile) and the mobile phase B (0.1% formic acid solution) were used in a gradient elution program. The target herbicides were detected by electrospray ionization mass spectrometry in positive ion mode with multi reaction monitoring (MRM). The result showed good linear relationships in the range of 0.5 ~ 20 ng/mL and the correlation coefficients were all above 0.99. The recoveries at spiked levels of 5, 10, 20 μg/kg for all target compounds in the samples were at the range of 62.7% ~ 117.4%, with relative standard deviations of 1.7% ~ 13.9%. The limits of detection (LOD, S/N ³ 3) for 20 pesticides were less than 0.63 μg/kg. The limits of quantitation (LOQ, S/N ³ 10) were less than 2.1 μg/kg. The method was specific, sensitive and accurate, and could be used for the determination of 20 triazine and sulfonylurea herbicides residues in foods of plant origin.
Template and target information: triazine herbicides, sulfonylurea herbicides
Author keywords: Composite molecular imprinted polymer solid phase-extraction, high performance liquid chromatography-mass spectrometry, Triazine herbicide, sulfonylurea herbicides