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Abstract: The Au-GR/GE sensor was fabricated for detection of Rutin by drop coating of graphene (GR) on GE (gold electrode) and electrodeposition of nanogold paticles (Au). Using rutin as template molecule, o-aminophenol as the functional monomer, the molecular imprinting polymer (MIP) electrochemical sensor which had a specific recognition to rutin was formed on the surface of Au-GR/GE through polymerization reaction. In this study, the performance, structure and function of MIP were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Quercetin which had a similar structure to rutin was used to compare the responses and the result showed that the sensor had a good selectivity of rutin. Under the optimized experimental conditions, the current response of the imprinted sensor was linear to the concentration of rutin in the range of 6.30 x 10-7 ~ 1.70 x 10-4 mol/L with linear equation as follows: I (μA) = 3. 8136 - 8.6247 lgc (mol/L), R = 0.9961, and a limit of detection was 2.10 x 10-7 mol/L. The sensor was used to determine the content of rutin in black tea samples, and the result was satisfactory.
Template and target information: rutin
Author keywords: Rutin, Electrodepodisition, Graphene nanogold composite film, molecular imprinting