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Reference type: Book Chapter
Authors: Canfarotta F, Smolinska-Kempisty K, Piletsky S
Publication date: 2017
Chapter title: Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection.
Page numbers: 389-398.
DOI: 10.1007/978-1-4939-6857-2_25

Book title: Synthetic Antibodies: Methods and Protocols
Editors: Tiller T
Publisher: Springer
City: New York, NY
ISBN: 978-1-4939-6857-2

Abstract: The enzyme-linked immunosorbent assay (ELISA) is a widely employed analytical test used to quantify a given molecule. It relies on the use of specific antibodies, linked to an enzyme, to target the desired molecule. The reaction between the enzyme and its substrate gives rise to the analytical signal that can be quantified. Thanks to their robustness and low cost, molecularly imprinted polymer nanoparticles (nanoMIPs) are a viable alternative to antibodies. Herein, we describe the synthesis of nanoMIPs imprinted for vancomycin and their subsequent application in an ELISA-like format for direct replacement of antibodies
Template and target information: vancomycin
Author keywords: Molecularly imprinted nanoparticles, Diagnostics, assay, Analyte quantification


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