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Reference type: Journal
Authors: Ma XT, He XW, Li WY, Zhang YK
Article Title: Epitope molecularly imprinted polymer coated quartz crystal microbalance sensor for the determination of human serum albumin.
Publication date: 2017
Journal: Sensors and Actuators B: Chemical
Volume: 246
Page numbers: 879-886.
DOI: 10.1016/j.snb.2017.02.137
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400517303647

Abstract: Quantitative determination of biomacromolecules has great significance. Quartz crystal microbalance sensors are favored by their sensibility while their selectivity was limited. Molecularly imprinted polymer has great selectivity performance. By combining the advantages of quartz crystal microbalance and molecularly imprinted polymer, this work established an epitope molecularly imprinted polymer based quartz crystal microbalance sensor. Using the epitope of human serum albumin as the template, the epitope molecularly imprinted polymer (EMIP) was successfully synthesized. Then a simple coating method was applied to fabricate the epitope molecularly imprinted polymer based quartz crystal microbalance sensor (EMIP-QCM). The EMIP-QCM sensor had good sensibility and selectivity to the target human serum albumin. The linear range was from 0.050 μg mL-1 to 0.500 μg mL-1 and the detection limit was calculated to be 0.026 μg mL-1. In addition, it could be used in real sample analysis and had good accuracy and reproducibility. This method proposed a general and simple way of establishing quartz crystal microbalance sensors for sensitive determination of biomacromolecules
Template and target information: peptide, epitope, protein, human serum albumin, HSA
Author keywords: Epitope imprinting, quartz crystal microbalance, Protein detection, human serum albumin


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