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Reference type: Journal
Authors: Zhang XY, Zhang N, Du CB, Guan P, Gao XM, Wang CY, Du YF, Ding SC, Hu XL
Article Title: Preparation of magnetic epitope imprinted polymer microspheres using cyclodextrin-based ionic liquids as functional monomer for highly selective and effective enrichment of cytochrome c.
Publication date: 2017
Journal: Chemical Engineering Journal
Volume: 317
Page numbers: 988-998.
DOI: 10.1016/j.cej.2017.02.138
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1385894717303182

Abstract: Epitope molecularly imprinted polymers (EMIPs) were synthesized using a novel cyclodextrin-based ionic liquid as functional monomer for specific recognition of cytochrome c (Cyt c) in aqueous solution via the combination of epitope and surface imprinting approach. Herein, the surface-exposed C-terminus of Cyt c (residues 96-104, AYLKKATNE) was selected as the template. Results reveal that the charged monomer cyclodextrins (CDs), namely mono-6A-deoxy-6-(1-vinylimidazolium)-β-cyclodextrin tosylate, could confer EMIPs with superior adsorption (67.6 mg g-1) and excellent recognition property (4.54) toward Cyt c due to its multiple binding sites with template such as hydrogen bonding, π-π effect, electrostatic, hydrophobic as well as steric effects. Moreover, the magnetic property of EMIPs has endowed the whole separation process with great convenience and efficiency, which demonstrated their potential application for large-scale separation and recognition
Template and target information: protein, peptide, epitope, AYLKKATNE, cytochrome c, Cyt c
Author keywords: Cyclodextrin-based ionic liquid, Epitope and surface imprinting, Multiple interactions, Magnetic property, Cytochrome C


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