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Reference type: Book Chapter
Authors: Sangma C, Lieberzeit PA, Sukjee W
Publication date: 2017
Chapter title: H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers.
Page numbers: 381-388.
DOI: 10.1007/978-1-4939-6857-2_24

Book title: Synthetic Antibodies: Methods and Protocols
Editors: Tiller T
Publisher: Springer
City: New York, NY
ISBN: 978-1-4939-6857-2

Abstract: Normally, antibodies against influenza A have been prepared from viable virus or an engineered strain in certain hosts or cultured media. Two factors concerning antibody production are obvious. The obtaining antibody that is a kind of biomolecule has to be handled carefully, e.g., to be kept in a refrigerator. Furthermore, when the virus strain is highly pathogenic, such as H5N1, antibody production has to be done carefully in a high-level biosafety lab. Here, we show how to produce an antibody against H5N1 from a polymeric material using inactivated virus which can be conducted in a low-level biosafety lab. The process is based on imprinting the whole virus on a polymer surface to form molecularly imprinted polymers (MIPs). The MIPs show some properties of H5N1 antibody as they recognize H5N1 and have some important antibody activity. The H5N1 MIPs are not to be considered biomaterial, so they can be stored at room temperature and thus do not need any special care
Template and target information: influenza virus, virus, H5N1 virus
Author keywords: plastic antibody, H5N1 influenza virus, Molecularly imprinted polymers, surface imprinting, H5N1 MIPs, Synthetic H5N1 antibody

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