Alternatively for mipdatabase quick searches go here

Custom Search

Abstract: As biomarkers of many diseases, glycoproteins are of great significance to clinical diagnostics. However, the determination of low abundant glycoproteins in complex biological samples without any pretreatment process is still a problem. In this study, a rapid and convenient separation method for highly efficient enrichment of glycoproteins is reported, based on pH double-responsive imprinted magnetic microspheres. Thin imprinted polymer shells were fabricated onto the surface of magnetic microspheres by free radical polymerization, using 2-(Dimethylamino) ethyl methacrylate as pH-sensitive monomer, 4-vinylphenylbronic acid as boronate affinity monomer, and ovalbumin (OVA) as template molecule. Combining the advantages of pH-sensitive monomer and boronate affinity monomer, rapidly capture-release of OVA could be modulated by changing solution pH. Moreover, high absorption ability (81.2 mg/g) was achieved within about 10 min. This study provided responsible way to imprint glycoproteins and showed great potential for glycoprotein detection in clinical diagnostic
Template and target information: protein, glycoprotein, ovalbumin, OVA
Author keywords: pH double-responsive, boronate affinity, glycoproteins, surface imprinting