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Reference type: Journal
Authors: Xie JF, Zhong GQ, Cai CQ, Chen CY, Chen XM
Article Title: Rapid and efficient separation of glycoprotein using pH double-responsive imprinted magnetic microsphere.
Publication date: 2017
Journal: Talanta
Volume: 169
Page numbers: 98-103.
DOI: 10.1016/j.talanta.2017.03.065
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914017303600

Abstract: As biomarkers of many diseases, glycoproteins are of great significance to clinical diagnostics. However, the determination of low abundant glycoproteins in complex biological samples without any pretreatment process is still a problem. In this study, a rapid and convenient separation method for highly efficient enrichment of glycoproteins is reported, based on pH double-responsive imprinted magnetic microspheres. Thin imprinted polymer shells were fabricated onto the surface of magnetic microspheres by free radical polymerization, using 2-(Dimethylamino) ethyl methacrylate as pH-sensitive monomer, 4-vinylphenylbronic acid as boronate affinity monomer, and ovalbumin (OVA) as template molecule. Combining the advantages of pH-sensitive monomer and boronate affinity monomer, rapidly capture-release of OVA could be modulated by changing solution pH. Moreover, high absorption ability (81.2 mg/g) was achieved within about 10 min. This study provided responsible way to imprint glycoproteins and showed great potential for glycoprotein detection in clinical diagnostic
Template and target information: protein, glycoprotein, ovalbumin, OVA
Author keywords: pH double-responsive, boronate affinity, glycoproteins, surface imprinting

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