Abstract: New molecularly imprinted polymers (MIPs) were prepared by using attapulgite (ATP) as the support, β-CD and methacrylic acid (MAA) as functional monomer, resveratrol as template molecule through surface molecularly imprinted polymerization technology. The MIPs were used as sorbent filled into the multiplug filtration clean-up (m-PFC) column for resveratrol enrichment and purification. A new method was developed for the determination of resveratrol by using multiplug filtration clean-up column with molecularly imprinted polymers extraction (MIPs-m-PFCE) combined with fluorescence spectrometry. The results showed that the present method has a high selectivity for RES under the optimized experimental conditions, the adsorption capacity of MIPs was 12.78 mg/g. The calibration curve was linear in the range of 0.1-100 μg/mL with a correlation coefficient (r) of 0.999 7. The limit of detection was 0.048 μg/L. The proposed method was successfully applied in the determination of RES in peanut root, with average recoveries of 86.2%-102.1% and relative standard deviations (RSDs) of 3.5%-5.8% at three spiked levels. The developed method is rapid and selective, and is adaptable to the analysis of trace RES in real samples.
Template and target information: resveratrol
Author keywords: resveratrol, molecular imprinted polymers, multiplug filtration clean-up column, molecular fluorescence spectrometry, peanut root