Abstract: Molecularly imprinted polymer (MIP) is widely used in many fields because of its high selectivity, chemical stability and easy preparation. Selectivity and extraction efficiency of the specific targets can be enhanced when MIP is coupled with solid-phase microextraction (SPME). In this work, a MIP-coated SPME fiber was prepared using oxytetracycline (OTC)as the template molecule and stainless steel wire modified by dopamine as the matrix. In addition, a method was developed for simultaneous extraction and determination of tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC) in milk and water samples by high performance liquid chromatography with a diode array detector (HPLC-DAD). 3mL of milk and water sample were extracted by the as-prepared fiber for 50 min, and then desorbed with mobile phase for 5min, respectively, and the desorption solution was then analyzed by HPLC-DAD. The results indicated that the extraction yield of the MIP-coated fibers was better than that of the non-imprinted polymer (NIP)coated fibers on the TCs. The linear correlation coefficients of TC, OTC and CTC were more than 0.9959 with the linear range of 100-1 000μg/L (milk) and 10-1 000μg/L (water), the limits of detection (S/N=3) for TC, OTC and CTC were 40-80μg/L (milk)and 5-10μg/L (water), and the recoveries of three TCs at a spiked concentration of 500μg/L were within the range of 97.8%-109.0% for milk and water samples with RSD (n=7)of 5.3%-8.2% (milk) and 3.7%-6.4% (water) restpectively. The results showed that this method was easy, green, selective, accurate and precise, and could be used to detect three TCs in milk and water samples.
Template and target information: tetracycline, TC, oxytetracycline, OTC, chlortetracycline, CTC
Author keywords: solid-phase microextraction, molecularly imprinted polymer, Tetracyclines, Water and milk