MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Book Chapter
Authors: Xu JJ, Medina-Rangel PX, Haupt K, Tse Sum Bui B
Publication date: 2017
Chapter title: Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis.
Chapter number: 6
Page numbers: 115-141.
DOI: 10.1016/bs.mie.2017.02.004 Alternative URL: http://www.sciencedirect.com/science/article/pii/S0076687917300514

Book title: NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes
Editors: Kumar CV
Publisher: Academic Press
ISBN: 0076-6879
Series title: Methods in Enzymology
Volume number: 590

Abstract: Molecularly imprinted polymers (MIPs) are synthetic antibody mimics possessing specific cavities designed for a target molecule. Nowadays, molecular imprinting of proteins still remains a challenge as the generation of selective imprinted cavities is extremely difficult, due to their flexible structure and the presence of a multitude of functional sites. To overcome this difficulty, we propose a solid-phase synthesis strategy to prepare MIPs specific for any protein that can be immobilized in an oriented way on a solid support. Trypsin and kallikrein were used as model proteins. The solid-phase support consists of glass beads functionalized with two affinity ligands of the enzymes, the competitive inhibitor p-aminobenzamidine to orient the enzymes via their active site, or a Cu2+ chelate to orient via the surface histidine residues of the enzyme. Thermoresponsive molecularly imprinted polymer nanoparticles (MIP-NPs) are then synthesized around the immobilized enzyme. The MIP-NPs are released by a simple temperature change, resulting in protein-free polymers endowed with improved binding site homogeneity since all binding sites have the same orientation. The MIP-NPs exhibit apparent dissociation constants between 0.02 and 2 nM toward their target proteins, which is comparable to those of natural antibodies. Moreover, these water-compatible polymers, targeting different domains of the enzyme, can also function as protective agents (armor), hence preventing the target proteins from denaturation by heat or pH
Template and target information: protein, trypsin, kallikrein
Author keywords: MIPs, solid-phase synthesis, protein imprinting, trypsin, Kallikrein, plastic antibodies, synthetic receptors, Metal chelate

  Shirt featuring the name Paulina spelled out in symbols of the chemical elements  Mug featuring the name Peter spelled out in the single letter amino acid code  We need to talk about Kelvin shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner