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Reference type: Journal
Authors: Gao WH, Liu B, Pang J, Li XF, Jia YM
Article Title: Determination of Bitertanol and Diniconazole in Food by Virtual-template Molecularly Imprinted Solid Phase Extraction-HPLC.
Publication date: 2013
Journal: Modern Food Science and Technology
Volume: 29
Issue: (10)
Page numbers: 2505-2508.
Alternative URL: http://www.xdspkj.cn/ch/reader/view_abstract.aspx?file_no=20131034&flag=1

Abstract: Myclobutanil molecularly imprinted polymer (MMIP) was prepared by bulk polymerization using myclobutanil as template molecule, methacrylate acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as crosslinker and acetonitrile as pore-foaming agent. The solid phase extraction (SPE) column was produced with MMIP as SPE agent. Fungicides of Bitertanol and diniconazole in food could be separated and enriched by SPE column, and then residuals were determined by HPLC. The sample was separated by the C18 column with a mobile phase of methanol and water (90:10, V/V) at the rate of 1mL/min and the wavelength was 210 nm. Their contents were quantified by external standard method. The average recoveries of bitertanol and diniconazole were 89.40~90.88% and 81.37~83.26%, respectively. The detection limits were all 0.20 μg/g. RSDs were 1.08~2.04% for bitertanol and 1.29~2.52% for diniconazole. In conclusion, the polymer had a good adsorption capacity for the two fungicides and the virtual template molecularly imprinted solid phase extraction technology could be used for the separation and detection of the two fungicides.
Template and target information: myclobutanil, bitertanol, diniconazole
Author keywords: bitertanol, Diniconazole, virtual template technology, solid phase extraction, high performance liquid chromatography

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