Abstract: Since the S-ovalbumin content in egg white has a significant correlation with the freshness and quality of chicken eggs, S-ovalbumin can be consider as a useful indicator for the same. Conditions were optimized using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) to prepare a molecularly-imprinted polypyrrole (MIPs) sensor that selectively recognizes S-ovalbumin. The optimal scan cycles, scan rate, template extraction time, and incubation time were 18 cycles, 100 mV/s, 1.5 h, and 40 min, respectively. The micro-structure of MIPs and non-MIPs were characterized by field emission scanning electron microscopy (FE-SEM). DPV was used to evaluate the dependency between different concentrations of S-ovalbumin and changes in peak current. The results showed that the linear response range of MIPs was from 10-6 to 10-3 mg/mL with a detection limit of 6.75 x 10-7 mg/mL. The MIPs exhibited a selective response to S-ovalbumin, and can therefore be used in the detection of S-ovalbumin.
Template and target information: protein, S-ovalbumin
Author keywords: Egg white, S-ovalbumin, polypyrrole, molecular imprinting, Electrochemical sensor