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Reference type: Journal
Authors: Chen G, Jin MJ, Du PF, Zhang C, Cui XY, Zhang YD, She YX, Shao H, Jin F, Wang SS, Zheng LF, Wang J
Article Title: A sensitive chemiluminescence enzyme immunoassay based on molecularly imprinted polymers solid-phase extraction of parathion.
Publication date: 2017
Journal: Analytical Biochemistry
Volume: 530
Page numbers: 87-93.
DOI: 10.1016/j.ab.2017.05.008
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0003269717302051

Abstract: The chemiluminescence enzyme immunoassay (CLEIA) method responds differently to various sample matrices because of the matrix effect. In this work, the CLEIA method was coupled with molecularly imprinted polymers (MIPs) synthesized by precipitation polymerization to study the matrix effect. The sample recoveries ranged from 72.62% to 121.89%, with a relative standard deviation (RSD) of 3.74-18.14%.The ratio of the sample matrix-matched standard curve slope rate to the solvent standard curve slope was 1.21, 1.12, 1.17, and 0.85 for apple, rice, orange and cabbage in samples pretreated with the mixture of PSA and C18. However, the ratio of sample (apple, rice, orange, and cabbage) matrix-matched standard-MIPs curve slope rate to the solvent standard curve was 1.05, 0.92, 1.09, and 1.05 in samples pretreated with MIPs, respectively. The results demonstrated that the matrices of the samples greatly interfered with the detection of parathion residues by CLEIA. The MIPs bound specifically to the parathion in the samples and eliminated the matrix interference effect. Therefore, the CLEIA method have successfully applied MIPs in sample pretreatment to eliminate matrix interference effects and provided a new sensitive assay for agro-products
Template and target information: parathion
Author keywords: chemiluminescence enzyme immunoassay, Matrix effect, Molecularly imprinted polymers, Parathion


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