Reference type: Journal
Authors: Sun GY, Liu YF, Ahat H, Shen AJ, Liang XM, Xue XY, Luo YQ, Yang J, Liu ZS, Aisa HA
Article Title: "Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract.
Publication date: 2017
Journal: Journal of Chromatography A
Volume: 1505
Page numbers: 35-42.
DOI: 10.1016/j.chroma.2017.05.033
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0021967317307380

Abstract: In this study, "two dimensional" molecularly imprinted solid-phase extraction (2D-MIP-SPE) of semi-preparative grade was constructed to fast purify ellagitannins in pomegranate husk extract with the help of crystallization and reverse-phase liquid chromatgoraphy (RPLC). Ellagic acid and punicalagin imprinted polymers were synthesized in batch mode and two semi-preparative MIP-SPE columns were individually packed. After investigaing "functional complementation", 2D-MIP-SPE was constructed using ellagic acid MIP and punicalagin MIP-SPE as the first and second dimension, respectively. Then, pomegranate husk extract was fast divided into four fractions individually enriching in ellagic acid, granatin A, punicalagin and ellagic acid glucoside by 2D-MIP-SPE. With the aid of crystallization and RPLC, ellagic acid (13.5 mg) and punicalagin (53.4 mg) were fast obtained in 30 min. Ellagic acid glucoside was purified to the purity near 100% with a recovery of 86.1%. Granatin A (92%) was directly obtained by 2D-MIP-SPE with the recovery of 81.8%. All above indicated that 2D-MIP-SPE was highly efficient in natural product purification. The concept of "functional complementation" was expected to be a useful tool in the construction of 2D-MIP-SPE
Template and target information: ellagic acid, punicalagin, ellagic acid glucoside, granatin A
Author keywords: "Two-dimensional" molecularly imprinted solid-phase extraction, Functional complementation, crystallization, high-performance liquid chromatography, Pomegranate husk extract