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Reference type: Journal
Authors: Peng DP, Li ZZ, Wang YL, Liu ZL, Sheng F, Yuan ZH
Article Title: Enzyme-linked immunoassay based on imprinted microspheres for the detection of sulfamethazine residue.
Publication date: 2017
Journal: Journal of Chromatography A
Volume: 1506
Page numbers: 9-17.
DOI: 10.1016/j.chroma.2017.05.016
Alternative URL: https://www.researchgate.net/publication/316783860_Enzyme-linked_immunoassay_based_on_imprinted_microspheres_for_the_detection_of_sulfamethazine_residue

Abstract: This study attempts to develop an enzyme-linked immunoassay (ELISA) using a molecularly imprinted polymer (MIP) as an artificial recognition element. The MIP microspheres were prepared using precipitation polymerization with SM2 as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. After the microspheres were coated in microtiter plate wells, the molecular imprinting ELISA (MI-ELISA) method was established based on the direct competition between free SM2 and horseradish peroxidase (HRP)-labelled SM2 in heterogeneous mode. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 100 μg L-1 - 3200 μg L-1. Furthermore, following the selective solid-phase extraction (SPE) with bulk SM2 MIPs as the sorbent and MI-ELISA detection, the limits of detection and quantification were 6.8 μg kg-1 and 20.4 μg kg-1, respectively, for SM2 in swine muscle. For the first time, MI-ELISA combined with molecular imprinting SPE was developed to determine trace SM2 in real samples, and the results show that it can be a useful analytical tool for quick detection in residue analysis
Template and target information: sulfamethazine, SM2
Author keywords: sulfamethazine, Molecular imprinting microsphere, Enzyme-linked assay


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