MIPs logo MIPdatabase      GSSMIP 2019; Deadline for abstract submission: 30th June 2019; Registration deadline: 19th July 2019
Custom Search
Reference type: Journal
Authors: Ribeiro JA, Pereira CM, Silva AF, Sales MGF
Article Title: Electrochemical detection of cardiac biomarker myoglobin using polyphenol as imprinted polymer receptor.
Publication date: 2017
Journal: Analytica Chimica Acta
Volume: 981
Page numbers: 41-52.
DOI: 10.1016/j.aca.2017.05.017
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0003267017306694

Abstract: An electrochemical biosensor was developed by merging the features of Molecular Imprinting technique and Screen-Printed Electrode (SPE) for the simple and fast screening of cardiac biomarker myoglobin (Myo) in point-of-care (POC). The MIP artificial receptor for Myo was prepared by electrooxidative polymerization of phenol (Ph) on a AuSPE in the presence of Myo as template molecule. The choice of the most effective protein extraction procedure from the various extraction methods tested (mildly acidic/basic solutions, pure/mixed organic solvents, solutions containing surfactants and enzymatic digestion methods), and the optimization of the thickness of the polymer film was carefully undertaken in order to improve binding characteristics of Myo to the imprinted polymer receptor and increase the sensitivity of the MIP biosensor. The film thickness was optimized by adjusting scan rate and the number of cycles during cyclic voltammetric electropolymerization of Ph. The thickness of the polyphenol nanocoating of only few nanometres (~4.4 nm), and similar to the protein diameter, brought in significant improvements in terms of sensor sensitivity. The binding affinity of MIP receptor film was estimated by fitting the experimental data to Freundlich isotherm and a ~8 fold increase in the binding affinity of Myo to the imprinted polymer (KF = 0.119 0.002 ng-1 mL) when compared to the non-imprinted polymer (KF = 0.015 0.002 ng-1 mL) which demonstrated excellent (re)binding affinity for the imprinted protein. The incubation of the Myo MIP receptor modified electrode with increasing concentration of protein (from 0.001 ng mL-1 to 100 μg mL-1) resulted in a decrease of the ferro/ferricyanide redox current. LODs of 2.1 and 14 pg mL-1 were obtained from calibration curves built in neutral buffer and diluted artificial serum, respectively, using SWV technique, enabling the detection of the protein biomarker at clinically relevant levels. The prepared MIP biosensor was applied to the determination of Myo spiked serum samples with satisfactory results
Template and target information: protein, myoglobin, Myo
Author keywords: biosensor, myoglobin, polyphenol, molecular imprinting, Screen-printed gold electrode, synthetic receptor

  Template of Doom spoof movie poster shirt  Chemistry peptide mug  Chemists are fun customisable shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner