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Reference type: Book Chapter
Authors: Zhang K, Zhou TC, Ye L, Bülow L
Publication date: 2017
Chapter title: Characterization of Protein-Protein Interactions in Recombinant Hemoglobin Producing Escherichia coli Cells Using Molecularly Imprinted Polymers.
Page numbers: 367-373.
DOI: 10.1007/978-3-319-55231-6_48

Book title: Oxygen Transport to Tissue XXXIX
Editors: Halpern HJ, LaManna JC, Harrison DK, Epel B
Publisher: Springer International Publishing
City: Cham
ISBN: 978-3-319-55231-6

Abstract: The worldwide blood shortage has generated demands for alternatives to transfusible human blood. One such important option is based on recombinant hemoglobin-based oxygen carriers (rHBOCs). Most efforts have been focused on various E. coli based production systems. One of the key challenges in these systems is to devise an efficient and economical protein production strategy involving selection of suitable host cell and Hb variant, growth conditions and media engineering. Hb also influences the heterologous host cell metabolism and therefore the identification of modified protein-protein interactions is critical for optimizing Hb production. In this study, molecularly imprinted polymers (MIPs) directed against Hb were used to identify the human Hb protein interaction network in E. coli. One E. coli host protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), interacted strongly with Hb, especially fetal Hb (HbF)
Template and target information: protein, hemoglobin, Hb
Author keywords: Recombinant hemoglobin, molecularly imprinted polymer, Glyceraldehyde 3-phosphate dehydrogenase, protein-protein interaction, Escherichia coli


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