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Reference type: Journal
Authors: Bakhshpour M, Tamahkar E, Andaç M, Denizli A
Article Title: Surface imprinted bacterial cellulose nanofibers for hemoglobin purification.
Publication date: 2017
Journal: Colloids and Surfaces B: Biointerfaces
Volume: 158
Page numbers: 453-459.
DOI: 10.1016/j.colsurfb.2017.07.023
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0927776517304368

Abstract: There is a significant need for the development of the novel adsorbents in the field of protein purification. In this study, thin hemoglobin imprinted film (MIP) was fabricated onto the bacterial cellulose nanofibers' (BCNFs) by surface imprinting method using metal ion coordination interactions with N-methacryloyl-(L)-histidinemethylester (MAH) and copper ions. The hemoglobin surface imprinted bacterial cellulose nanofibers (MIP-BCNFs) was applied to selective recognition of hemoglobin and purification from hemolysate. The characterization of the MIP-BCNFs was carried out by the Fourier Transformed Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), micro-Computerized Tomography (μCT) atomic force microscopy (AFM) and surface area measurements. The adsorption experiments of hemoglobin onto the MIP-BCNFs and NIP-BCNFs from aqueous hemoglobin solutions were investigated in a batch system. The results showed that MIP-BCNFs are promising materials for purification of hemoglobin with high adsorption capacity, significant selectivity and reusability
Template and target information: protein, hemoglobin
Author keywords: Hemoglobin purification, surface imprinting, Metal ion coordination, bacterial cellulose nanofibers


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