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Reference type: Journal
Authors: Wei YB, Zeng Q, Hu Q, Wang M, Tao J, Wang LS
Article Title: Self-cleaned electrochemical protein imprinting biosensor basing on a thermo-responsive memory hydrogel.
Publication date: 2018
Journal: Biosensors and Bioelectronics
Volume: 99
Page numbers: 136-141.
DOI: 10.1016/j.bios.2017.07.049
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566317305079

Abstract: Herein, the self-cleaned electrochemical protein imprinting biosensor basing on a thermo-responsive memory hydrogel was constructed on a glassy carbon electrode (GCE) with a free radical polymerization method. Combining the advantages of thermo-responsive molecular imprinted polymers and electrochemistry, the resulted biosensor presents a novel self-cleaned ability for bovine serum albumin (BSA) in aqueous media. As a temperature controlled gate, the hydrogel film undergoes the adsorption and desorption of BSA basing on a reversible structure change with the external temperature stimuli. In particular, these processes have been revealed by the response of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) of electroactive [Fe(CN)6]3-/4-. The results have been supported by the evidences of scanning electron microscopy (SEM) and contact angles measurements. Under the optimal conditions, a wide detection range from 0.02 μmol L-1 to 10 μmol L-1 with a detection limit of 0.012 μmol L-1 (S/N = 3) was obtained for BSA. This proposed BSA sensor also possesses high selectivity, excellent stability, acceptable recovery and good reproducibility in its practical applications
Template and target information: protein, bovine serum albumin, BSA
Author keywords: Molecularly imprinted polymers, Electrochemical biosensor, Bovine serum albumin, Thermo-responsive memory hydrogel


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