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Reference type: Journal
Authors: Pathak A, Parveen S, Gupta BD
Article Title: Ultrasensitive, highly selective, and real-time detection of protein using functionalized CNTs as MIP platform for FOSPR-based biosensor.
Publication date: 2017
Journal: Nanotechnology
Volume: 28
Issue: (35)
Page numbers: ArticleNo355503.
DOI: 10.1088/1361-6528/aa79e5
Alternative URL: http://stacks.iop.org/0957-4484/28/i=35/a=355503

Abstract: A facile approach is presented for the detection of bovine serum albumin (BSA), based on fiber optic surface plasmon resonance (FOSPR) combined with molecular imprinting (MI). The probe is fabricated by exploiting the plasmonic property of silver thin film and vinyl-functionalised carbon nanotube-based MIP platform. BSA template molecules are imprinted on the MIP layer coated over multi-walled carbon nanotubes to ensure high specificity of the probe in the interfering environments. In addition, FOSPR endorses the sensor capability of real-time and remote sensing along with very high sensitivity due to the use of nanostructured MI platform. The response of the probe is considered in terms of the absorbance spectrum recorded for various concentrations of BSA. The sensor shows a wide dynamic range of 0-350 ng l-1 with a considerably linear response up to 100 ng l-1 in the peak absorbance wavelength with BSA concentration. A highest sensitivity of 0.862 nm per ng l-1 is achieved for the lowest concentration of BSA and it decreases with the increase in BSA concentration. The performance of the present sensor is compared with those reported in the literature in terms of the limit of detection. It is found that the probe possesses a lowest LOD of 0.386 ng l-1 in addition to other advantages such as real-time online monitoring, high sensitivity, high specificity, and remote sensing
Template and target information: protein, bovine serum albumin, BSA

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