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Abstract: A novel fluorescence probe was designed and synthesized to quantify ractopamine in foods using molecularly imprinted polymer (MIP) as a specific recognition element and YF3:Yb3+, Er3+ upconversion nanoparticles (UCNPs) as a fluorescence signaling component. The developed UCNP@MIP probe was characterized using X-ray powder diffraction, scanning electron microscopy, and thermogravimetric analysis. The fluorescence of UCNP@MIP probe was quenched specifically by ractopamine, and good linear regression ranging from 1.66 x 10-8 to 3.3 x 10-7 mol/L was obtained with the limit of detection of 8.6 x 10-10 mol/L. This method was further applied to determine ractopamine in water and pork samples. Recoveries between 76.61 and 88.97% were obtained with relative standard deviation ranging from 1.15 to 2.67% (n = 3). This UCNP@MIP probe combined high selectivity of MIP and high sensitivity of upconversion fluorophore and showed potential to determine various food chemical hazards
Template and target information: ractopamine
Author keywords: Upconversion nanoparticles, molecularly imprinted polymer, Fluorescent probe, Ractopamine