MIPdatabase
Authors: Lian ZR, Wang JT
Article Title: Selective isolation of gonyautoxins 1,4 from the dinoflagellate Alexandrium minutum based on molecularly imprinted solid-phase extraction.
Publication date: 2017
Journal: Marine Pollution Bulletin
Volume: 122
Issue: (1)
Page numbers: 500-504.
DOI: 10.1016/j.marpolbul.2017.06.066
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0025326X17305635
Abstract: Gonyautoxins 1,4 (GTX1,4) from Alexandrium minutum samples were isolated selectively and recognized specifically by an innovative and effective extraction procedure based on molecular imprinting technology. Novel molecularly imprinted polymer microspheres (MIPMs) were prepared by double-templated imprinting strategy using caffeine and pentoxifylline as dummy templates. The synthesized polymers displayed good affinity to GTX1,4 and were applied as sorbents. Further, an off-line molecularly imprinted solid-phase extraction (MISPE) protocol was optimized and an effective approach based on the MISPE coupled with HPLC-FLD was developed for selective isolation of GTX1,4 from the cultured A. minutum samples. The separation method showed good extraction efficiency (73.2-81.5%) for GTX1,4 and efficient removal of interferences matrices was also achieved after the MISPE process for the microalgal samples. The outcome demonstrated the superiority and great potential of the MISPE procedure for direct separation of GTX1,4 from marine microalgal extracts
Template and target information: gonyautoxins 1,4, GTX1,4, dual template
Author keywords: Gonyautoxins 1,4, Alexandrium minutum, molecularly imprinted solid-phase extraction, Double-templated imprinting
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