MIPdatabase
Authors: Zhang C, Cui HY, Han YF, Yu FF, Shi XM
Article Title: Development of a biomimetic enzyme-linked immunosorbent assay based on molecularly imprinted polymers on paper for the detection of carbaryl.
Publication date: 2018
Journal: Food Chemistry
Volume: 240
Page numbers: 893-897.
DOI: 10.1016/j.foodchem.2017.07.109
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0308814617312621
Abstract: A biomimetic enzyme-linked immunosorbent assay (BELISA) which was based on molecularly imprinted polymers on paper (MIPs-paper) with specific recognition was developed. As a detector, the surface of paper was modified with γ-MAPS by hydrolytic action and anchored the MIP layer on γ-MAPS modified-paper by copolymerization to construct the artificial antibody Through a series of experimentation and verification, we successful got the MIPs-paper and established BELISA for the detection of carbaryl. The development of MIPs-paper based on BELISA was applied to detect carbaryl in real samples and validated by an enzyme-linked immunosorbent assay (ELISA) based on anti-carbaryl biological antibody. The results of these two methods (BELISA and ELISA) were well correlated (R2=0.944). The established method of MIPs-paper BELISA exhibits the advantages of low cost, higher stability and being re-generable, which can be applied as a convenient tool for the fast and efficient detection of carbaryl
Template and target information: carbaryl
Author keywords: molecularly imprinted polymer, Biomimetic enzyme-linked immunosorbent assay, Paper, Carbaryl, enzyme-linked immunosorbent assay
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