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Reference type: Journal
Authors: Wang XY, Yu JL, Li JH, Kang Q, Shen DZ, Chen LX
Article Title: Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins.
Publication date: 2018
Journal: Sensors and Actuators B: Chemical
Volume: 255
Issue: (Part 1)
Page numbers: 268-274.
DOI: 10.1016/j.snb.2017.08.068
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400517314909

Abstract: Molecular imprinting as a versatile technology has gained popularity in various fields for diverse species; however, protein imprinting still remains challenging due to some inherent problems but is potentially rewarding work. Herein, we reported a facile strategy to imprint phycocyanin (PC), as a template, using quantum dots (QDs) co-capped by thioglycollic acid and glutathione directly as functional monomer via dopamine self-polymerization, which effectively simplified the imprinting process and afforded easy accessibility to recognition sites. The resulting QDs based imprinting nanosensor showed significant fluorescence decrease of QDs within less than 16 s upon binding PC owing to the ultrathin imprinting layer (ca. 3 nm), leading to a high detectability down to 0.075 μM. An excellent linearity was found within the wide range of 0.8-8.0 μM (R2 = 0.9935). Good recognition selectivity toward PC was displayed over other possible competing molecules, with a high imprinting factor of 7.3. Seawater and lake water samples spiked with PC were also analyzed, presenting satisfactory recoveries ranging from 90.8-110.1% with precisions below 4.6%, indicating the practicality of the imprinting nanosensor for accurately rapid nanoscale monitoring of algae blooms. By using another protein, bovine haemoglobin (BHb), as a convincing support model, the present study can provide a simple and general imprinting strategy toward various concerned proteins through rationally utilizing diverse functionalization and interactions
Template and target information: protein, phycocyanin, PC
Author keywords: molecular imprinting, protein imprinting, fluorescent detection, Phycocyanin, Quantum dots

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