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Abstract: A novel glycoprotein imprinting strategy was proposed and was applied to the detection of the carbohydrate antigen 19-9. The glycosylated complex of glycoprotein was used as template to construct a glycosyl imprinted sensor. The eluted imprinted cavities showed good affinity for template glycosyls and glycoproteins carrying template glycosyl. The effect of template saccharide structure on glycosyl imprinted sensors is further discussed. More complex template structures can lead to better sensor performance including selectivity and sensitivity. As a result, the polysaccharide imprinted sensor showed preeminent linear response to CA19-9 in the range of 0.1-5U/mL, with a detection limit of 0.028U/mL (3δ /K), while the linear of the monosaccharide imprinted sensor was 1-60U/mL and the detection limit was 0.17U/mL. The complex structure on the template surface provides more possibilities for the recognition of the template molecules, consequently, led to the significant anti-interference capability of the polysaccharide imprinted sensor. Furthermore, recoveries ranging from 93.0% to 103.5% were achieved when human serum samples were assayed using the polysaccharide imprinted sensor
Template and target information: polysaccharide, monosaccharide, glycosyl, glycoprotein, protein
Author keywords: macromolecular imprinting, Glycosyl imprinting, CA19-9, polysaccharide, Monosaccharide, Tumor marker