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Reference type: Journal
Authors: Saylan Y, Tamahkar E, Denizli A
Article Title: Recognition of lysozyme using surface imprinted bacterial cellulose nanofibers.
Publication date: 2017
Journal: Journal of Biomaterials Science-Polymer Edition
Volume: 28
Issue: (16)
Page numbers: 1950-1965.
DOI: 10.1080/09205063.2017.1364099

Abstract: Here, we developed the lysozyme imprinted bacterial cellulose (Lyz-MIP/BC) nanofibers via the surface imprinting strategy that was designed to recognize lysozyme. This study includes the molecular imprinting method onto the surface of bacterial cellulose nanofibers in the presence of lysozyme by metal ion coordination, as well as further characterizations methods FTIR, SEM and contact angle measurements. The maximum lysozyme adsorption capacity of Lyz-MIP/BC nanofibers was found to be 71 mg/g. The Lyz-MIP/BC nanofibers showed high selectivity for lysozyme towards bovine serum albumin and cytochrome c. Overall, the Lyz-MIP/BC nanofibers hold great potential for lysozyme recognition due to the high binding capacity, significant selectivity and excellent reusability
Template and target information: protein, lysozyme, Lyz
Author keywords: bacterial cellulose nanofibers, Lysozyme recognition, Metal ion coordination, Protein purification, surface imprinting


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