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Abstract: A new molecularly imprinted electrochemical sensor for direct detection of insulin was prepared based on epitope imprinting. The molecularly imprinted polymer was fabricated by electropolymerization with C-terminal polypeptide of insulin self-assembled on the Au electrode as template molecule and o-PD as functional monomer. After elution of template molecules by NaOH solution, the imprinting cavities were formed with the three-dimensional structure matching with the C-terminal polypeptide of insulin molecule. The imprinting cavities could specifically recognize and rebind with insulin molecules. With K3[Fe(CN)6]/K4[Fe(CN)6] as a probe, the insulin could be indirectly detected. There was a linear relationship between the response current and the concentration of insulin in the range of 1.0 x 10-14 - 5.0 x 10-13 M, and the detection limit was 7.24 x 10-15 M (3σ/S). The developed sensor exhibited good selectivity and stability; and was successfully applied to determination of insulin in serum samples with satisfactory results
Template and target information: peptide, epitope, C-terminal polypetide of insulin, insulin
Author keywords: Insulin, molecular imprinting, Electrochemical sensor, Epitope imprinting