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Abstract: Objective To establish a preparation method of molecularly imprinted polymer monolithic column (MIP-MC) with the high cross-linking structures, and determine the tetracyclines residues in milk powder by on-line solid-phase extraction coupled with high performance liquid chromatography (SPE-HPLC). Methods The MIP-MC was prepared in a stainless steel column using oxytetracycline as the template, methacrylic acid and hydroxyethyl methacrylate as functional monomers, ethylene glycol dimethacrylate and dipentaerythritol hexaacrylate as the cross-linking agent, azobisisobutyronitrile as initiator, and acetone-methanol-dodecanol as porogenic solvent. The obtained monolith was applied to the determination of tetracycline drugs residues in milk powder by SPE-HPLC. Results An enrichment factor of 19.3 along with a good sample clean-up effect was obtained under optimum on-line solid phase extraction conditions. The recoveries of tetracyclines at 0.05, 0.25 and 0.5 mg/kg were in the range of 84.2%~103.4% with the relative standard deviation of 1.37%~4.87%. The limits of detection (S/N=3) and quantitation (S/N=10) of the proposed methods were 8.48~11.74 mg/kg and 28.24~39.09 mg/kg, respectively. Conclusion The on-line solid phase extraction method is simple, rapid and highly sensitive, and it is suitable for the determination of tetracyclines antibiotics residues in milk powder.
Template and target information: oxytetracycline, tetracyclines