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Reference type: Journal
Authors: Suo DC, Wang RG, Wang PL, Fan X, Su XO
Article Title: Pseudo template molecularly imprinted polymer for determination of 14 kind of β-agonists in animal urine by ultra-high-performance liquid chromatography-tandem mass spectrometry.
Publication date: 2017
Journal: Journal of Chromatography A
Volume: 1526
Page numbers: 23-30.
DOI: 10.1016/j.chroma.2017.09.076
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0021967317314577

Abstract: A pseudo template molecularly imprinted polymer (MIP) for selective extraction of β-agonists was prepared using 4-hydroxyphenethyl alcohol as pseudo template molecule, methyl acrylic acid as functional monomer, and ethylene glycol dimethacrylate as cross-linker. Selectivity and adsorption capacity of prepared MIP were studied in detail. MIP selectively adsorbed 14 types of β-agonists. Advantages of the prepared polymer include non-template leakage in purification of target analytes and cheap cost. MIP was used to purify β-agonists in urine samples. The developed method was combined with ultra-performance liquid chromatography-tandem mass spectrometry. In optimum conditions, recoveries of the developed method ranged from 84.2% to 109.8% with low coefficients of variation at below 15%. Limits of detection and quantification for 14 target β-agonists in urine samples reached below 0.05 ng/mL and 0.1 ng/mL, respectively. The developed method based on MIP enrichment and purification were successfully applied to analysis of target analytes in 50 actual urine samples
Template and target information: 4-hydroxyphenethyl alcohol, pseudo template, β-agonists
Author keywords: β-Agonists, Pseudo template molecularly imprinted materials, Ultraperformance liquid chromatography tandem mass spectrometry, pharmaceutical analysis


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