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Reference type: Journal
Authors: Liang GH, Zhai HY, Huang L, Tan XC, Zhou Q, Yu X, Lin HD
Article Title: Synthesis of carbon quantum dots-doped dummy molecularly imprinted polymer monolithic column for selective enrichment and analysis of aflatoxin B1 in peanut.
Publication date: 2018
Journal: Journal of Pharmaceutical and Biomedical Analysis
Volume: 149
Page numbers: 258-264.
DOI: 10.1016/j.jpba.2017.11.012
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0731708517319313

Abstract: In this study, a novel method was proposed to sensitively determine aflatoxin B1 (AFB1) in peanut sample by using a carbon quantum dots-coated dummy molecularly imprinted polymer (CDs-DMIP) monolithic column for pretreatment coupled with high performance liquid chromatography-fluorescence detection (HPLC-FLD). The CDs-DMIP monolithic column was prepared by in-situ polymerization in a water bath using 5,7-dimethoxycoumarin as dummy template molecule. The CDs-DMIP monolithic column was applied to determine AFB1 by HPLC-FLD. Satisfactory linearity was obtained over 0.5-2000 ng mL-1, with a high correlation coefficient of 0.9999. The recoveries of AFB1 in peanut sample ranged from 79.5% to 91.2%, and the intraday and interday relative standard deviation ranged from 1.2% to 4.9%. Limit of detection (S/N = 3) and limit of quantitation (S/N = 10) were 0.118 ng mL-1 and 0.393 ng mL-1, respectively. Under the optimized conditions, the enrichment factor was over 71-fold. AFB1 in peanut sample and even some other samples could be sensitively determined by CDs-DMIP-HPLC-FLD method
Template and target information: 5,7-dimethoxycoumarin, dummy template, aflatoxin B1, AFB1
Author keywords: Aflatoxin B1, Molecularly imprinted monolithic columns, Carbon quantum dots, high performance liquid chromatography


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