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Reference type: Journal
Authors: Liu HL, Gong CC, Liao ZX, Chen T, Li YL
Article Title: Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC.
Publication date: 2017
Journal: Chromatographia
Volume: 80
Issue: (11)
Page numbers: 1651-1658.
DOI: 10.1007/s10337-017-3406-z

Abstract: An efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by 1H-NMR and 13C-NMR
Template and target information: caffeic acid, p-coumaric acid, ferulic acid
Author keywords: molecularly imprinted solid-phase extraction, HSCCC, Clematis akebioides, caffeic acid, separation

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