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Authors: Kubo T, Arimura S, Naito T, Sano T, Otsuka K
Article Title: Competitive ELISA-like Label-free Detection of Lysozyme by Using a Fluorescent Monomer-doped Molecularly Imprinted Hydrogel.
Publication date: 2017
Journal: Analytical Sciences
Volume: 33
Issue: (11)
Page numbers: 1311-1315.
DOI: 10.2116/analsci.33.1311
Alternative URL: https://www.jstage.jst.go.jp/article/analsci/33/11/33_1311/_article
Abstract: We report on a molecularly imprinted hydrogel for the selective fluorescent detection of a targeting protein. To achieve the fluorescent detection of proteins, a fluorescent functional monomer was synthesized with fluorescein isothiocyanate and allylamine. The monomer (FITC-AA) provided a gradable alteration of the absorption spectrum, and a decrease of the fluorescent intensity due to an interaction with the model protein, lysozyme. Also, the imprinted hydrogel (PI-gel) was simply prepared with FITC-AA, the anionic functional monomers, poly(ethylene glycol) dimethacrylate, and lysozyme by radical photo polymerization. The PI-gel showed selective adsorption for lysozyme and the imprinting factor reached to 2.7. Additionally, the PI-gel indicated a gradual decrease of the fluorescent intensity corresponding to the lysozyme concertation similar to a competitive enzyme-linked immunosorbent assay detection
Template and target information: protein, lysozyme
Author keywords: hydrogel, protein imprinting, fluorescent detection, lysozyme, competitive ELISA
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