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Reference type: Journal
Authors: Wang J, Sang YX, Liu WH, Liang N, Wang XH
Article Title: The development of a biomimetic enzyme-linked immunosorbent assay based on the molecular imprinting technique for the detection of enrofloxacin in animal-based food.
Publication date: 2017
Journal: Analytical Methods
Volume: 9
Issue: (47)
Page numbers: 6682-6688.
DOI: 10.1039/C7AY02321A

Abstract: In this study, a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method using molecularly imprinted polymers (MIPs) as artificial antibodies was developed to detect enrofloxacin (ENRO) in animal-based food. The MIPs were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, adsorption kinetic experiments and isothermal adsorption experiments. The results showed that molecularly imprinted polymers were successfully synthesized with a fast adsorption rate and high adsorption. Under the optimum conditions, the sensitivity (IC50) and limit of detection (IC15) of the established BELISA method were 85.7 ng mL-1 and 1.1 ng mL-1, respectively. The method was applied to the determination of ENRO in spiked beef and shrimp samples, and recoveries ranged from 74.4 to 86.8% with relative standard deviations (for n = 3) between 3.3 and 8.0%. The results correlated well with those obtained using ELISA and high performance liquid chromatography methods
Template and target information: enrofloxacin, ENRO

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