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Reference type: Journal
Authors: Pan MF, Gu Y, Zhang MY, Wang JP, Yun YG, Wang S
Article Title: Reproducible Molecularly Imprinted QCM Sensor for Accurate, Stable, and Sensitive Detection of Enrofloxacin Residue in Animal-Derived Foods.
Publication date: 2018
Journal: Food Analytical Methods
Volume: 11
Issue: (2)
Page numbers: 495-503.
DOI: 10.1007/s12161-017-1020-1

Abstract: This study describes the development of a novel reproducible molecularly imprinted quartz crystal microbalance (QCM) sensor for the accurate and sensitive analysis of the residue of enrofloxacin (ENRO) in animal-derived foods. This proposed sensor was easily fabricated by directly immobilizing molecularly imprinted polymer (MIP) of ENRO on the surface of a QCM Au chip, which combined the advantages of selective recognition from the MIP with the high sensitivity and portability of a QCM sensor. The parameters in the fabrication and measurement process were optimized and discussed in detail. It was verified that the MIP-modified QCM Au chip performed favorably for the detection of ENRO residue in common animal-derived food products and demonstrated acceptable accuracy (recovery: pure milk 77.2-84.2%, egg 77.3-85.6%, chicken muscle 73.5-89.1%, pork 74.7-85.8%), precision (relative standard deviation (RSD, n = 3), pure milk 2.9-8.0%, egg 2.9-6.4%, chicken muscle 3.4-6.8%, pork 2.2-4.7%), and sensitivity (limit of detection, pure milk 0.31 μg L-1, egg 0.44 μg kg-1, chicken muscle 0.54 μg kg-1, pork 0.57 μg kg-1). The MIP-modified QCM Au chip for sensing ENRO was portable, could be stored for an extended period of time, and reused for more than 30 analysis cycles with a response attenuation of 7.8%. These results have demonstrated that the proposed MIP QCM sensor presents an accurate, sensitive, rapid, and low-cost methodology for ENRO residue detection in animal foods. This research is very promising for the development of novel effective devices applied to the detection of various contaminants in the field of food safety
Template and target information: enrofloxacin, ENRO
Author keywords: Enrofloxacin, Molecularly imprinted QCM sensor, Animal-derived foods


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