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Reference type: Journal
Authors: Liu QR, Jiang MD, Ju ZL, Qiao XG, Xu ZX
Article Title: Development of direct competitive biomimetic immunosorbent assay based on quantum dot label for determination of trichlorfon residues in vegetables.
Publication date: 2018
Journal: Food Chemistry
Volume: 250
Page numbers: 134-139.
DOI: 10.1016/j.foodchem.2017.12.079
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0308814617320514

Abstract: A direct competitive biomimetic immunosorbent assay method based on molecularly imprinted polymer was developed for the determination of trichlorfon. A CdSe/ZnS quantum dot label was used as the marker. The hydrophilic imprinted film was synthesized directly on the surface of a 96-well plate, and characterized by Fourier-transform infrared spectroscopy and thermo-gravimetric analyses. The method exhibited high stability, selectivity, and sensitivity. Under optimal conditions, the limits of detection and sensitivity of the biomimetic immunosorbent assay method were 9.0 μg L-1 and 5.0 mg L-1 (0.1 mg kg-1 and 62.5 mg kg-1 for vegetable sample), respectively. Low cross-reactivity values of 19.2% and 15.6% were obtained for the structural analogues. Spinach and rape samples spiked with trichlorfon were extracted and determined by this method with recoveries ranging from 83.6% to 91.1%. The method was applied for the detection of trichlorfon residues in leek and cucumber samples, and results correlated well with those obtained using GC
Template and target information: trichlorfon
Author keywords: molecular imprinting, hydrophilic imprinted film, Biomimetic immunosorbent assay, Quantum dots


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