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Reference type: Journal
Authors: Zhang H, Wang PM, Zhou Q, Wang YH
Article Title: A Novel Method for the Detection of Chlorpyrifos by Combining Quantum Dot-labeled Molecularly Imprinted Polymer with Flow Cytometry.
Publication date: 2018
Journal: Analytical Letters
Volume: 51
Issue: (6)
Page numbers: 921-934.
DOI: 10.1080/00032719.2017.1364744
Alternative URL: https://www.researchgate.net/publication/319224625_A_Novel_Method_for_the_Detection_of_Chlorpyrifos_by_Combining_Quantum_Dot_Labeled_Molecularly_Imprinted_Polymer_with_Flow_Cytometry

Abstract: Uniform-sized fluorescent molecularly imprinted polymers were prepared by one-step swelling and suspension polymerization, while chlorpyrifos, methacrylic acid, ethylene glycol dimethacrylate, and oil-soluble CdSe/ZnS quantum dots were used as the carrier, template molecule, functional monomer, cross-linker, and fluorophor, respectively. The morphology, adsorption dynamics, binding ability, and selectivity of quantum dot-labeled molecularly imprinted polymers were evaluated. The dosage of quantum dots for labeling the molecularly imprinted polymers was optimized. The results showed that the optimized dose of quantum dots was 200?-ÁL using a concentration of 8.0?-ÁM. The microsphere size was approximately 10?-Ám with a honeycombed surface. The quantum dot-labeled molecularly imprinted polymers had an even brightness and a high selectivity. In the presence of different concentrations of chlorpyrifos, a decrease in the fluorescence intensity of the quantum dot-labeled molecularly imprinted polymer was clearly identified by flow cytometry. The whole detection process was accomplished within 2?h including pretreatment. This method was used for the determination of chlorpyrifos in tap water samples
Template and target information: chlorpyrifos
Author keywords: Chlorpyrifos, energy-dispersive X-ray analysis, flow cytometry, fluorescence, gas chromatography, Molecularly imprinted polymers, Quantum dots, Quenching, scanning electron microscopy


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