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Abstract: A new analytical method was developed involving a drug imprinted polymer according to polymerization with N-vinyl caprolactam as a thermo-sensitive monomer, allylacetoacetate as a functional monomer, N, N'-methylenebisacrylamide as a cross-linker agent, gemcitabine as a template and azobis isobutyronitrile as an initiator. The effect of time, pH and temperature on adsorption of gemcitabine has been investigated. Some techniques such as Fourier transform infrared spectroscopy, thermogravimetric analysis, elemental analysis and scanning electron microscopy were used for determination of drug imprinted polymer. The drug imprinted polymer was used as a solid-phase extractant to determine trace amount of gemcitabine in biological human fluids. It is found that the drug imprinted polymer sorbent extracted gemcitabine selectively. The profile of gemcitabine uptake with the new sorbent showed good accessibility of the reactive sites in the imprinted polymer sorbent. From the mathematical calculation, the equilibrium adsorption data of gemcitabine by imprinted polymer were evaluated by Langmuir, Freundlich and Temkin isotherm models. The introduced method was successfully used for determination of gemcitabine in pharmaceutical and human urine samples by high performance liquid chromatography with great results
Template and target information: gemcitabine
Author keywords: Gemcitabine, Human biological fluids, molecularly imprinted polymer, solid phase extraction, thermosensitive polymer