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Reference type: Journal
Authors: Li QJ, Jiang LD, Kamra T, Ye L
Article Title: Synthesis of fluorescent molecularly imprinted nanoparticles for turn-on fluorescence assay using one-pot synthetic method and a preliminary microfluidic approach.
Publication date: 2018
Journal: Polymer
Volume: 138
Page numbers: 352-358.
DOI: 10.1016/j.polymer.2018.01.086
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0032386118301149

Abstract: Fluorescent molecularly imprinted polymers (MIPs) have received considerable attention for their promising applications in sensing, imaging and diagnostics. Different synthetic methods have been reported to prepare fluorescent MIPs. Here, one-pot polymerization method was developed to synthesize fluorescent MIP nanoparticles (NPs) bearing a suitable fluorescent label as the fluorescence acceptor. The MIP NPs can emit strong fluorescence based on fluorescence resonance energy transfer (FRET) when they bind to analytes acting as the fluorescence donor. The one-pot synthetic method is straightforward, less time-consuming and easy to optimize the loading of fluorescence acceptor. Additionally, a preliminary microfluidic reactor was designed for preparing such fluorescent MIP NPs with more uniform size in a continuous process for the first time. The fluorescent MIP NPs can be used for selective detection of the analytical target in a FRET-based turn-on fluorescence assay which is easy to carry out with no tedious separation steps
Author keywords: molecularly imprinted polymer, One-pot synthesis, Fluorescence resonance energy transfer

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