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Reference type: Journal
Authors: Weber P, Riegger BR, Niedergall K, Tovar GEM, Bach M, Gauglitz G
Article Title: Nano-MIP based sensor for penicillin G: Sensitive layer and analytical validation.
Publication date: 2018
Journal: Sensors and Actuators B: Chemical
Volume: 267
Page numbers: 26-33.
DOI: 10.1016/j.snb.2018.03.142
Alternative URL: https://www.sciencedirect.com/science/article/pii/S0925400518306403

Abstract: We herein report the synthesis of novel Penicillin G (PenG) imprinted polymer nanoparticles (MIPs) via inverse miniemulsion polymerization. Nanoscaled co-polymer particles consisting of N-(2-aminoethyl) methacrylamide hydrochloride as functional monomer and N,N'-Ethylenebisacrylamide as crosslinker have been synthesized in the presence of PenG. These particles have been applied to form a sensitive layer for label-free direct optical sensing of Penicillin G. As reference material non-imprinted particles (NIPs) were used. The particles were characterized via scanning electron microscopy (SEM) and dynamic light scattering (DLS). Particles in the size of 400 nm (z-average) and a low polydispersity index (PDI < 0.05) were observed. Azide modified MIPs/NIPs were covalently immobilized on alkyne-modified glass transducers by Cu(I) catalyzed 1,3-dipolar cycloaddition. The resulting particle-modified transducers served as sensing layer in an optical sensor setup (Reflectomteric Interference Spectroscopy - RIfS). To prove its reliability and stability the transducer was tested in 78 reproducible PenG measurements over the course of 26 h. The response time of the sensor was 1 min. For sensor calibration 14 randomized triplicate concentration dependency measurements for MIP and NIP transducers were conducted with different PenG concentrations ranging from 0.0015-0.0195 mol/L. MIP binding signals were significantly higher compared to the NIP. Determined recovery rates of three different transducers were in the range of 70-120 % which indicates a good chip to chip reproducibility. Sensor cross sensitivities between PenG and its structural buildings blocks phenylacetic acid and 6-aminopenicillanic acid were evaluated indicating a high selectivity for the presented sensor system
Template and target information: penicillin G, PenG
Author keywords: Molecularly imprinted polymer nanoparticles, Inverse miniemulsion polymerization, Reflectometric Interference Spectroscopy (RIfS), molecular interactions, recognition elements, Penicillin G

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