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Reference type: Journal
Authors: Sari E, Üzek R, Duman M, Denizli A
Article Title: Detection of ciprofloxacin through surface plasmon resonance nanosensor with specific recognition sites.
Publication date: 2018
Journal: Journal of Biomaterials Science-Polymer Edition
Volume: 29
Issue: (11)
Page numbers: 1302-1318.
DOI: 10.1080/09205063.2018.1457417

Abstract: The novel nanosensor based on Surface Plasmon Resonance (SPR) was developed for sensitive and selective detection of ciprofloxacin via molecularly imprinted nanoparticles (MIP/NPs). NPs were synthesized through miniemulsion polymerization technique with methacrylic acid as a functional monomer. FTIR, SEM, zetasizer and contact angle measurements were used for the characterization of MIP/NPs. After modification the SPR chip surface, the nanosensor was used for detection of ciprofloxacin in aqueous solution. According to selected concentration range, the correlation coefficient and limit of detections were obtained as 0.993 (R2) and 3.21 and 7.1 ppb in ultrapure water and SWW, respectively. Association kinetic analysis, Scatchard, Freundlich, Langmuir and Freundlich-Langmuir isotherms were also performed on the data to investigate adsorption behaviour of ciprofloxacin on the surface of nanosensor. Tetracycline and enrofloxacin were used as competitor agents to examine the selectivity of nanosensor. Performance of the SPR nanosensor was also investigated by using synthetic wastewater (SWW) for detection of CPX. The reusability of nanosensor was investigated and good repeatability was obtained with 5.81% RSD. As a result, selective, simple and low-cost method to detect ciprofloxacin in aqueous solution was developed by combining MIP/NPs and SPR
Template and target information: ciprofloxacin, CPX
Author keywords: Ciprofloxacin, Nanosensor, surface plasmon resonance, molecular imprinting, miniemulsion


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