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Abstract: Bovine serum albumin (BSA) imprinted calcium alginate (CaAlg MIP) hydrogel film was prepared using BSA as template molecule, sodium alginate (NaAlg) as the functional monomer, and CaCl2 as the ionic cross-linker. The thickness of the film was controlled by a glass rod enlaced with brass wires. The relationship between copper wire diameter and the thickness of CaAlg MIP was investigated. SEM, laser scanning confocal microscope and 3D ultra-depth three-dimensional microscope were used to characterize the CaAlg MIP. The mechanical properties of the wet CaAlg MIP prepared with different concentrations of NaAlg were investigated. Circular dichroism (CD) was used to characterize the conformation of the eluted BSA. The influence of different thickness and different eluants on the adsorption capacity of CaAlg MIP was discussed. The results showed that CaAlg MIP adsorbed more BSA than CaAlg NIP. There was no change of the conformation of protein during the preparing and eluting process. The CaAlg MIP can be prepared by a fast, low-cost and environmental friendly method in aqueous solution in the absence of any toxic surfactant or organic solvent, which would find its potential applications in the fields of cell culture and tissue engineering.
Template and target information: protein, bovine serum albumin, BSA
Author keywords: calcium alginate hydrogel film, Bovine serum albumin, molecular imprinting, adsorption, thickness control