Alternatively for mipdatabase quick searches go here

Custom Search

Abstract: The homemade 6-APA molecular imprinted solid-phase extraction (MISPE) column was applied in the extraction and separation of penicillins in market milk, largely enhancing the analytical speed. The molecularly imprinted polymers (MIPs) was prepared using 6-aminopenicillanic acid which is the intermediate of penicillins as the virtual template, methylacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linking monomer. The structure and morphology of molecularly imprinted polymers were characterized by Fourier Transform infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The result of FTIR showed that there was hydrogen bonding sites inside of MIPs. The result of SEM indicated that there were large amounts of holes on the surface of MIPs. The selective adsorption ability of MIPs was detected by specific adsorption experiment, and it illustrated that the MIPs had selectivity for Penicillin drugs. The solid phase extraction (SPE) column packed with MIPs was used for pretreatment of milk sample, coupled with HPLC detection. The results came out that one sample contained high content of 6-APA, without other three PENs. The recovery experiment was conducted at 3 different spiked levels, and the results showed the recovery rate was 83.5% ~ 87.3%. The 6-APA MISPE column also has broad prospects in the detection of complex matrices like animal food, water samples and soil samples.
Template and target information: 6-aminopenicillanic acid, 6-APA, penicillins
Author keywords: intermediate, virtual template, molecularly imprinted solid-phase extraction, Penicillin antibiotic, milk