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Abstract: A novel molecularly imprinted polymer was developed by a one-step polymerization using dicyandiamide as template, methacrylic acid as functional monomer, ethylene glycol dimethylacrylate as cross-linker, and azobisisobutyronitrile as initiator. The physicochemical properties of the prepared material were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and ultraviolet spectroscopy. A molecularly imprinted solid phase extraction coupled with high performance liquid chromatography method was developed using this material to detect dicyandiamide in real milk powder samples. The linear range of this method was from 0.01 to 2.00 mg/L, with a correlation coefficient (R2) equal to 0.999 4, which had a good linear relationship. The limit of detection (LOD) was 0.57 x 10-2 mg/L (RSN = 3). The recovery was ranged from 102.5% to 104%, and the relative standard deviation was between 0.21% and 0.86%.
Template and target information: dicyandiamide
Author keywords: Dicyandiamide, molecularly imprinted polymers (MIP), Solid-phase extraction, high performance liquid chromatography (HPLC)