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Reference type: Journal
Authors: Wang SM, Jiang MD, Ju ZL, Qiao XG, Xu ZX
Article Title: A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon.
Publication date: 2018
Journal: Food and Agricultural Immunology
Volume: 29
Issue: (1)
Page numbers: 159-170.
DOI: 10.1080/09540105.2017.1364707

Abstract: In this study, a molecularly imprinted polymer (MIP) was synthesized using trichlorfon as a template molecule. The MIP exhibited a high adsorption capacity toward trichlorfon. Using bovine serum albumin as a carrier, trichlorfon hapten was indirectly labeled with luminol, which competed with free trichlorfon for the combination with a biomimetic antibody of MIP. Based on this direct competitive format, a high throughput flow-injection chemiluminescent biomimetic immunoassay method was developed for fast detection of trace trichlorfon. The influencing factors were investigated and optimized. Under the optimal condition, a lower limit of detection (IC15) of 0.0024 mg/L was obtained using this method. This method was also used to detect the trichlorfon spiked in carrot and cabbage samples, and the results were also verified using the method of gas chromatography
Template and target information: trichlorfon
Author keywords: flow injection, chemiluminescence, molecularly imprinted polymer, biomimetic immunoassay


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