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Reference type: Journal
Authors: Hong SH, She YX, Cao XL, Wang M, Zhang C, Zheng LF, Wang SS, Ma XB, Shao H, Jin MJ, Jin F, Wang J
Article Title: Biomimetic enzyme-linked immunoassay based on a molecularly imprinted 96-well plate for the determination of triazophos residues in real samples.
Publication date: 2018
Journal: RSC Advances
Volume: 8
Issue: (37)
Page numbers: 20549-20556.
DOI: 10.1039/C8RA03531H

Abstract: In this study, a direct competitive biomimetic enzyme-linked immune-sorbent assay (BELISA) based on a molecularly imprinted nanomembrane as an artificial antibody was developed for the determination of triazophos in real samples. The imprinted film was directly synthesized on the well surface of a 96-well plate using a dummy molecular template under the conditions of thermal polymerization. The developed BELISA using a hapten of triazophos as an enzyme-labeled probe is much more sensitive, simple, quick, steady and inexpensive than the other instrumental and immuno assay methods. Under optimal conditions, the linear range of the method was 0.001-10 000 μg L-1 with a good regression coefficient of 0.977. The sensitivity (IC50) and the limit of detection (LOD) of BELISA were 428 μg L-1 and 0.001 μg L-1, respectively. This method was performed to detect triazophos in cabbage and apple samples, and showed excellent recovery and relative standard deviations (RSDs) ranging from 70.5 to 119.8% and from 5.2 to 19.7%, respectively. The results correlated well with those obtained using high performance liquid chromatography-tandem mass spectrometry
Template and target information: triazophos


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