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Reference type: Journal
Authors: Grenier CJ, Timberman A, Yang RF, Csoros J, Papantones A, Deravi LF, Seitz WR
Article Title: Rapid, High Affinity Binding by a Fluorescein Templated Copolymer Combining Covalent, Hydrophobic, and Acid-Base Noncovalent Crosslinks.
Publication date: 2018
Journal: Sensors
Volume: 18
Issue: (5)
Page numbers: ArticleNo1330.
DOI: 10.3390/s18051330
Alternative URL: http://www.mdpi.com/1424-8220/18/5/1330

Abstract: A new type of biomimetic templated copolymer has been prepared by reverse addition fragmentation chain transfer polymerization (RAFT) in dioxane. The initial formulation includes the template fluorescein, N-isopropylacrylamide (NIPAM, 84 mol %), methacrylic acid (MAA, 5-mol %), 4-vinylpyridine (4-VP, 9 mmol %), and N,N'-methylenebis(acrylamide) (MBA, 2 mol %). PolyNIPAM is a thermosensitive polymer that comes out of aqueous solution above its lower critical solution temperature forming hydrophobic "crosslinks". MAA and 4-VP interact in dioxane forming acid–base crosslinks. The excess 4-VP serves as a recognition monomer organizing around the template fluorescein to form a binding site that is held in place by the noncovalent and covalent crosslinks. The MBA is a covalent crosslinker. The RAFT agent in the resulting copolylmer was reduced to a thiol and attached to gold nanoparticles. The gold nanoparticle bound copolymer binds fluorescein completely in less than two seconds with an affinity constant greater than 108 M-1. A reference copolymer prepared with the same monomers by the same procedure binds fluorescein much more weakly
Template and target information: fluorescein
Author keywords: templates, moleculary imprinted polymers, Poly(N-Isopropylacrylamide), noncovalent crosslinks, fluorescein, binding affinity, Binding kinetics

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